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Yan, Y. (2014). Cryopreservation of Pluripotent Stem Cell-Derived Neural Progenitor Aggregates Labelled with Micron-Sized Particles of Iron Oxide. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-9271
Magnetic resonance imaging (MRI) provides an effective approach to track the labeled pluripotent stem cell (PSC)-derived neural progenitor cells (NPCs) for neural transplantation and neurological disorder treatments. However, labeling the thawed cells after cryopreservation can be limited by the inefficient intracellular labeling and variations in labeling efficiency. Therefore, cryopreservation of the pre-labeled cells can provide uniform cell population and operational convenience for the following in vitro and in vivo investigations. In this study, the feasibility of cryopreserving PSC-derived NPC aggregates labeled with micron-sized particles of iron oxide (MPIO) was investigated. The NPC aggregates derived from embryoid body formation were labeled in suspension with different concentrations of MPIO in the range of 0-100 ug Fe per mL. The results indicated that intracellular MPIO incorporation was retained after cryopreservation (70-80% labeling efficiency), which did not significantly affect cell recovery, proliferation, cytotoxicity and neural lineage commitment. MRI analysis was performed in the phantom tissue environment using cell layers with different MPIO exposures separated by agarose gels. The results showed comparable detectability for the MPIO-labeled cells before and after cryopreservation indicated by T2 and T2* relaxation rates. These findings indicate the feasibility of cryopreserving MPIO-labeled PSC-derived NPC aggregates for potential cell banking toward various in vitro and in vivo cell tracking studies.
Cryopreservation, Iron oxide particle, Magnetic resonnance imaging, Neural progenitor aggregate, Pluripotent stem cell
Date of Defense
November 5, 2014.
Submitted Note
A Thesis submitted to the Department of Chemical and Biomedical Engineering in partial fulfillment of the requirements for the degree of Master of Science.
Bibliography Note
Includes bibliographical references.
Advisory Committee
Yan Li, Professor Directing Thesis; Samuel C. Grant, Committee Member; Jingjiao Guan, Committee Member.
Publisher
Florida State University
Identifier
FSU_migr_etd-9271
Use and Reproduction
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Yan, Y. (2014). Cryopreservation of Pluripotent Stem Cell-Derived Neural Progenitor Aggregates Labelled with Micron-Sized Particles of Iron Oxide. Retrieved from http://purl.flvc.org/fsu/fd/FSU_migr_etd-9271