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Riskowski, R. A. (R. A. ). (2016). Theory and Applications of Surface Energy Transfer for 2-20 Nm Diameter Metal Nanoparticles. Retrieved from http://purl.flvc.org/fsu/fd/FSU_2016SU_Riskowski_fsu_0071E_13408
The development and experimental validation of a mathematical model for nanoparticle–based surface energy transfer (SET) between gold nanoparticles and fluorescent dye labels, has enabled biophysical studies of nucleic acid structure and function previously inaccessible by other methods. The main advantages of SET for optical distance measurements are that it can operate over longer distances than other similar methods, such as Forster Resonance Energy Transfer (FRET), thus enabling measurements across biological structures much larger than otherwise possible. This work discusses the fundamental theory for the SET interaction and expansion of SET theory to account for multiple interacting dye labels and demonstrated on DNA and RNA in order to allow 3D triangulation of labeled structures. SET theory has also been expanded to core@shell structures which represent a new class of designer SET platforms with dramatically increased spectral windows; allowing for a multitude of dye labels to be used simultaneously over a broad range of wavelengths. Additionally, these designer nanostructures can incorporate the material properties of the core. So that , for example Ni@Au, can provide a SET measurement platform coupled with a magnetic moment for sample purification and manipulation. These efforts to develop and establish optical SET methods lays a foundation of a powerful methodology for biophysical characterization, and allows researchers to study biological structures previously too large or complex to be easily studied, such as the unknown tertiary structures of large RNA elements.
DNA, Energy Transfer, Nanoparticles, Optical Ruler, RNA
Date of Defense
June 28, 2016.
Submitted Note
A Dissertation submitted to the Institute of Molecular Biophysics in partial fulfillment of the requirements for the degree of Doctor of Philosophy.
Bibliography Note
Includes bibliographical references.
Advisory Committee
Geoffrey F. Strouse, Professor Directing Dissertation; Richard L. Hyson, University Representative; Piotr G. Fajer, Committee Member; M. Elizabeth Stroupe, Committee Member; Hank W. Bass, Committee Member.
Publisher
Florida State University
Identifier
FSU_2016SU_Riskowski_fsu_0071E_13408
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Riskowski, R. A. (R. A. ). (2016). Theory and Applications of Surface Energy Transfer for 2-20 Nm Diameter Metal Nanoparticles. Retrieved from http://purl.flvc.org/fsu/fd/FSU_2016SU_Riskowski_fsu_0071E_13408