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Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
Hydrogen/Deuterium Exchange Monitored by Liquid Chromatorgaphy Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry—Method Optimization and Applications to the Study of Protein Conformation and Protein—Drug Interactions
Proteins are the most versatile macromolecules in the cell. Major biochemical functions of proteins include binding; catalysis; and serving as structural components of cells and organisms. Proteins are made of amino acids that are...
High-Performance Liquid Chromatography Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry of Citrus Extract: Structural Probing of Flavonoids
For fragmentation studies whole citrus peel extracts were obtained from organic Florida-grown Valencia oranges. The application of positive-ion Electrospray Ionization (ESI) Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT...
Limiting undesired interactions of proteins with surfaces is a vital task for implementation of many technologies that require direct exposure to protein media. This includes sensors, single molecule spectroscopy studies, and...
Zinc ions (Zn2+) perform significant roles in biological processes. Therefore, the detection and imaging of Zn2+ in biological systems are of great interest. In this thesis, several 1, 2, 3-triazolyl-containing N4-tetradentate ligands and...
Liquid chromatography is a popular, analytical technique that is utilized in countless industries. With problems and samples becoming more and more complex, it is important to maximize the separation. In traditional cation-exchange...
The work described in this thesis details the development of a quintuple-detector SEC separation to measure chemical heterogeneity and determine the solution conformation for copolymers and polymer blends. Chemical heterogeneity affects...
This dissertation is divided into two parts. In the first part, phosphorus sequestration in wetland soils is linked to carbon humification. The goal of the second part is to provide molecular-level characterization of biochars. The...
Contrary to the tremendous amount of information on genomes and proteomes, coverage on lipidomes is limited. Polar lipids, for example, glycolipids and phospholipids, play important roles in cellular functions and not just provide...
Central to the advancement of reversed-phase high-performance liquid chromatography (RPLC) is to develop new synthetic strategies for manufacturing stationary phase materials. Methods to improve the efficiency, retention properties, and...
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) interference is a newly discovered small-RNA based immune system used by bacteria and archaea. One of the essential steps in this interference is to process long CRISPR...
Biocompatible, nonporous and stoichiometric polyelectrolyte blends of both poly(styrene sulfonate), PSS, and poly(diallyldimethylammonium), PDADMA, were produced for the first time on a scale of ca. 300 g h-1 using a laboratory extruder....
It has been known for 35 years that phase correction of Fourier transform ion cyclotron resonance (FT-ICR) mass spectral data can in principle produce an absorption-mode spectrum with mass resolving power as much as a factor of 2 higher...
The use of gas chromatography-mass spectrometry to measure the relative pool size and 13C-enrichment of a broad range of intracellular metabolites to monitor changes in the metabolome of mammalian cells is described. The typical workflow...
ABSTRACT The development of easily-accessible and densely-functionalized synthetic intermediates is a longstanding goal of the organic chemistry community. The use of transition metal catalysts has allowed many synthetic targets to be...
Size-Exclusion Chromatography with Refractometric and Viscometric Detection to Determine the Solution Conformational Entropy of Mono-, Di-, and Oligosaccharides
The solution flexibility of carbohydrates in general, and of oligosaccharides in particular, influences a variety of biological processes, such as binding strength, molecular recognition, and even disease prevention. For monosaccharides, ...
Size-exclusion chromatography (SEC) is a separation technique commonly used for size and molar mass analysis. The primary mechanism of separation by SEC is not by enthalpic interactions, like in such techniques as gas chromatography (GC)...
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) interference is a newly discovered small-RNA based immune system used by bacteria and archaea. One of the essential steps in this interference is to process long CRISPR...
Hydrogen/Deuterium Exchange Monitored by Liquid Chromatorgaphy Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry—Method Optimization and Applications to the Study of Protein Conformation and Protein—Drug Interactions
Proteins are the most versatile macromolecules in the cell. Major biochemical functions of proteins include binding; catalysis; and serving as structural components of cells and organisms. Proteins are made of amino acids that are...
Eventually, the world will deplete the global oil supply and a new form of energy will supplant fossil fuels as the main global energy source. However, technological advances are still decades away from finding a unified solution to meet...
Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
