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Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
With the advent of two "soft" ionization techniques in the late 1980s—electrospray ionization (ESI) and matrix assisted laser desorption/ionization (MALDI) for the routine and general formation of intact molecular ions—and continuing...
This work focuses on the development of new synthetic methods for the preparation of various N-heterocycles. While I have encountered many road blocks throughout my PhD, it has taught me how to solve a wide variety of challenges through...
Chromatography, as a separation technique, has received wide acceptance and achieved widespread application. Reversed-phase liquid chromatography (RPLC) is a versatile separation mode utilized in biotechnology, pharmaceutical and other...
The work in this dissertation presents microfluidic platforms developed for the evaluation of islet function. Pancreatic islets of Langerhans release glucagon and insulin to maintain blood glucose levels, and the release of these...
Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) provides the highest mass resolving power and mass measurement accuracy for confidant identification of biomolecules such as proteins and peptides. Chapter 1 briefly...
The Poisson-Boltzmann equation, PBE, the linearized PBE, LPBE, and generalized Born, GB, model are implicit solvent methods that accelerate biophysical calculations by eliminating the need to integrate across the solvent's degrees of...
Prebiotic processes required a reliable source of free energy and complex chemical mixtures that likely included sugars. The formose reaction is a potential source of those sugars but is tied to alkaline conditions and elevated...
Olfactory perception affects the food choice of most living species. A strong set of data have been accumulated to demonstrate that the olfactory bulb, the first relay of olfactory information, as a metabolic sensor. Mitral cells of the...
Structural biology is a field studying the relationship between structure and function of macro biomolecules, including proteins. Proteins with numerous different functions are synthesized by recruiting amino acids into a linear...
The work in this dissertation presents methods for sensitive and selective assays for hormones and proteins involved in the regulation of glucose homeostasis. The capillary electrophoresis (CE) conditions for a competitive immunoassay of...
With the development of ionization techniques such as electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI), mass spectrometry expands its subject of study from small volatile molecules to non-volatile...
The Iron-dependent Regulator (IdeR) is a 230-amino acid transcriptional repressor that regulates iron homeostasis, oxidative stress response and virulence in Mycobacterium tuberculosis. IdeR binds to DNA as a dimer of dimers to repress...
Many of the genes that regulate eye and retinal development have been identified based upon mutant analysis in animal models or human diseases. Taking advantage of the mosaic organization of the photoreceptor in zebrafish our laboratory...
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