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Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
This work describes the activation profiles and regulatory cascades of the human kallikrein-related peptidases. The human kallikrein (KLK)-related peptidases are the largest family of serine peptidases, comprising 15 members (KLK1–15)...
In eukaryotes and archaea 5-25% of transfer RNA (tRNA) precursors contain intervening sequences, or introns, that interrupt the molecules' functional regions. Because functional tRNA molecules are necessary for protein synthesis, ...
A generalized ensemble based enhanced sampling scheme, the Orthogonal Space Sampling (OSS) approach, is introduced in this thesis. Due to the fact that the OSS scheme simultaneously accelerates the motions of a target event and its...
Nature presents active components in the form of proteins and molecular motors that are involved in locomotion and inter compartmental transport. Inspired by nature, this dissertation focuses on presenting experimental means to...
The scope of this dissertation is two-fold: assembly of histidine-tagged peptides and proteins directly to the surface of a 1.5 nm AuNP and the assembly and delivery of nano-medical materials for MR imaging and gene therapy. Chapter 1 is...
The Iron-dependent regulator (IdeR) protein is a member of the diphtheria toxin repressor (DtxR) family of metal responsive proteins that regulates genes involved in iron homeostasis, oxidative stress response, and virulence in...
Helical membrane proteins have been difficult targets for structural characterization but are ~30% of the typical genome. This dissertation is focused on the extension of solid state nuclear magnetic resonance methodology to be able to...
The Baldwin rules provide a robust guideline for predicting the favorability of ring closure of reactive intermediates based upon stereoelectronic considerations. Our group was intrigued by the lack of examples of 5-endo-dig cyclizations...
The RNA splicing and processing endonuclease from Nanoarchaeum equitans (NEQ) belongs to the recently identified (ab)2 family of splicing endonucleases that require two different subunits for splicing activity. N. Equitans splicing...
Arginine kinase (AK; arginine phosphate + MgADP + H+ "²"³ arginine + MgATP) and creatine kinase (CK; creatine phosphate + MgADP + H+ "²"³ creatine + MgATP) play a key role in energy homeostasis in cells that display high and variable...
The use of gas chromatography-mass spectrometry to measure the relative pool size and 13C-enrichment of a broad range of intracellular metabolites to monitor changes in the metabolome of mammalian cells is described. The typical workflow...
Gene therapy is a rising field and requires multifunctional delivery platforms in order to overcome the cellular barriers. Quantum dots (QDs) provide a optically fluorescent and biocompatible surface to act as a multifunctional delivery...
Studies of 1,2,3-Triazoles: Synthesis, Post-Synthetic Funcationalization and Application in Fluorescent Sensors for the Intracellular Detection of Zinc Ions
1, 2, 3-Triazoles are important targets for drug design due to their similarities to amides. The bioisosteric replacement of amides and other less inert functional groups with 1, 3-triazoles has proven to be highly successful due to their...
The Iron-dependent Regulator (IdeR) is a 230-amino acid transcriptional repressor that regulates iron homeostasis, oxidative stress response and virulence in Mycobacterium tuberculosis. IdeR binds to DNA as a dimer of dimers to repress...
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