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Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
Type II diabetes is a leading cause of death in the United States due to its high prevalence in adults with high blood pressure, obesity, other health problems. It affects ~10.5% of the U.S population, and this number continues to...
In this dissertation, the work presented describes an integrated microfluidic system composed of pressure driven perfusion and online measurement techniques for analyses of hepatic metabolism. The liver is primarily made up of...
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) offer an adaptive immune system that protects bacteria and archaea from nucleic acid invaders through an RNA-mediated nucleic acid cleavage mechanism. Our knowledge of...
Breast cancer is the second leading death-causing cancer in women, after lung cancer. Breast cells are normally responsive to the estrogen hormone as they have the receptor for it. Breast cancer types that express the estrogen receptor...
The properties of proteins with reduced sequence complexity--either in the form of primary structure symmetry or restricted amino acid alphabets--are controversial. Studies of protein biophysics and sequence analyses suggest that...
There is a well-established relationship between the olfactory and the endocrine systems. At the center of this relationship sits the olfactory bulb, which acts as a relay station for the sense of smell. In fact, neurons in the olfactory...
The work in this dissertation presents a method for multiplexing fluorescence measurements in both polymerase chain reaction (PCR) and anisotropy. For PCR, two dual color infrared mediation assays were demonstrated on a microfluidic...
CrgA is a small helical membrane protein from Mycobacterium tuberculosis (Mtb) and known to recruit other cell division proteins to the divisome, a substantial protein complex for Mtb cell division. There is a lack of information about...
This dissertation aims to advance the understanding and applicability of nanotechnology through the use of nanoparticles as conjugated delivery agents. By providing multi-functionality of a nanoparticle and a nucleic acid therapeutic, ...
Murine adenosine deaminase (ADA), a 40 kDa enzyme, catalyzes the hydrolytic deamination of adenosine to form inosine, enhancing the rate of the spontaneous reaction by more than than 1011 times the rate of the non-catalyzed reaction....
Changes in environmental conditions cause alterations in the metabolism of cells. The field of metabolomics seeks to assay for and understand on a whole-cell level how metabolism responds to these changes. In this dissertation, a method...
The work described in this thesis details the development of a quintuple-detector SEC separation to measure chemical heterogeneity and determine the solution conformation for copolymers and polymer blends. Chemical heterogeneity affects...
CRISPR Cas9 is a bacterial immune system which has been found to be extremely useful for the purposes of genomic editing and DNA detection methods. While it is possible to "program" Cas9 proteins for specific target sites, any site must...
The innate immune system acts as the first line of defense against invading pathogens throughrecognition of pathogen associated molecular patterns or danger associated molecular patterns. Several germline encoded receptors, known as the...
Free fatty acids (FFAs) are signaling molecules secreted by adipocytes, or fat cells. Individual FFAs differ in their ability to stimulate or attenuate insulin secretion depending on the chain length and degree of unsaturation of the FFA...
Insect flight muscle (IFM) is the preferred model system for visualizing actin-myosin interactions due to its highly ordered lattice of actin and myosin filaments. Electron tomography (ET) of fast-frozen, actively contracting Lethocerus...
Found throughout nature, science, industry, and medicine, conjugated dienes and trienes are ubiquitous. While their behavior changes upon substitution, they are all built from the same fundamental units of either 1, 3-butadiene or the 1, 3...
Myosin V, as a member of the myosin family, can "walk" along F-actin to transport many "cargos", such as mRNA and secretary vesicles, to its destination. This study is part of a research project that aims to understand the interaction...
The Clustered Regularly Interspaced Short Palindromic Repeat loci found in most archaea and some bacteria contain DNA sequences (spacers) that originate from genetic invaders like viruses, transposons, and plasmids. The CRISPR clusters...
In the United States, breast cancer is the most commonly diagnosed cancer in women, except for skin cancers. The American Cancer Society estimates there will be 268, 600 new cases in 2019 alone. It is also the second leading cause of...
The work in this dissertation presents a microfluidic method for the quantitative measurement of insulin secretion rates and patterns from single pancreatic islets of Langerhans. Proper release of insulin from islets is essential for...
Signaling through the notch pathway originates upon interaction between the notch receptor and one of its two ligand proteins, delta or serrate. Extracellular binding of either ligand protein induces the release of the notch...
Mass spectrometry, featured at high sensitivity, is an indispensable tool in the field of biophysics for studying intact protein and protein complex. Fourier Transform Ion Cyclotron Resonance (FTICR) mass spectrometer offers unparalleled...
The introduction of cryo-electron microscopy (cryo-EM) has greatly improved our understanding of structural biology, especially regarding muscle tissue. This technique has been key in revealing the complex structure and functional...
Ribosomes are giant RNA-protein complexes responsible for translating the information encoded by the genome to create the proteome in all kingdoms of life. In budding yeast, the mature cytoplasmic 80S ribosome consists of 79 ribosomal...
Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
