Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
Type II diabetes is a leading cause of death in the United States due to its high prevalence in adults with high blood pressure, obesity, other health problems. It affects ~10.5% of the U.S population, and this number continues to...
Petroleum, one of the most complex mixtures on Earth, has been extensively studied for decades. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) and the ultrahigh resolution it provides, however, has dramatically...
In this dissertation, the work presented describes an integrated microfluidic system composed of pressure driven perfusion and online measurement techniques for analyses of hepatic metabolism. The liver is primarily made up of...
Society today is still very dependent on petroleum, the most complex naturally occurring mixture on the planet. Ultrahigh resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) has been employed to...
High resolution mass spectrometry, especially Fourier transform ion cyclotron resonance mass spectrometry (FT ICR MS) is a widely practiced technique of choice in proteomics and lipidomics due to its high sensitivity, reproducibility and...
Manual Nuclear Magnetic Resonance (NMR) spectral analysis of proteins is a time intensive effort with methods often specific to each analysis. The method described in this thesis automates the resonance assignment of protein side chains...
Characterization of Emerging Contaminants from Weathered Fossil Fuel-Derived Materials as Revealed by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS)
Fossil fuel-derived materials are readily incorporated into the built environment without much knowledge about how they can impact the long-term well-being of natural ecosystems and public health. Ultrahigh resolution Fourier transform...
Most biomolecular NMR experiments rely on 1H detection because it offers high sensitivity, but 1H NMR experiments are limited by the chemical shift dispersion of 1H which presents significant challenges for accurate peak identification...
Polyelectrolytes are polymers consisting of charged repeat units resulting in a net charge for the macromolecules. Mixing oppositely charged polyelectrolytes creates polyelectrolyte complexes (PEC). Different types of complexes can be...
Cashews are the number one produced tree nut worldwide and the U.S. is the top importer. Despite their popularity and economic importance, cashews are frequently implicated in IgE-mediated food allergy. Food allergy is an increasingly...
Hydrogen is an element with increasing importance as we shift towards green and renewable energy sources. As such, the physical and chemical properties of hydrogen and materials which help store or produce hydrogen are necessary for the...
The work in this dissertation presents a chiral separation method for the quantitative measurement of primary amines secreted from murine islets of Langerhans along with whole brain tissue and astrocytes. Primary amines, including amino...
From inside our bodies to the environment we live in, there are a wide variety of unknown compounds that have been difficult to identify and characterize through current methods. We aim to study these compounds by liquid chromatography ...
The work in this dissertation presents microfluidic platforms developed for the evaluation of islet function. Pancreatic islets of Langerhans release glucagon and insulin to maintain blood glucose levels, and the release of these...
Mass spectrometry (MS) has become an important analytical method for proteomic research due to its high mass accuracy, resolution, and selectivity. Even though the traditional bottom-up MS-based method is still a widespread routine for...
In this dissertation, liquid chromatography and solid phase extraction – mass spectrometry (LC and SPE-MS) techniques are applied for rapid quantitation of four glucose-regulating hormones secreted from human islets of Langerhans. The...
In this dissertation, fluorescence and microfluidics-based methods to study biological dynamics of pancreatic islets of Langerhans are described. The approaches discussed are all able to house biological tissues and keep them viable...
The work in this dissertation presents methods for study of glucose stimulated insulin secretion as well as other hormones involved in glucose homeostasis. A microfluidic system was developed to investigate the entrainment of insulin...
Characterization of Protein Sequence Variants and Posttranlational Modifications by Top-down Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Histone variants and post-translational modifications (PTMs) are known to play central roles in genome regulation and maintenance. Quantitavely characterization of the variants and the associated PTMs is the first step to understand...
One of the grand challenges in the field of human health is to understand the structure-function relationship of biological molecules. To date, there exist many types of low and high resolution methods for interrogating the structure of...
Membrane proteins contribute about 30% of the proteome and about 60% of the drugs in the market target membrane proteins. But only about 1.5% of structures in Protein Data Bank are membrane proteins which reflect the challenges in...
The work in this dissertation presents microfluidic methods developed for the study of biological dynamics. The requirements for the methods development was to create approaches with the ability to perform dynamic cell stimulation, ...
Pyrolysis oil has shown potential as an environmentally-friendly petroleum replacement for the production of fuel and chemicals. Also, the use of waste materials in the production of pyrolysis oil alleviates concerns associated with...
This work summarizes the development of several novel separation techniques and characterization methods for petroleum products. The first section summarizes the development of a new method for screening petroleum crude oils for the...
Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
